Flight attendant performing aircraft disinsection by spraying insecticide aerosols in an airplane cabin, following international health protocols.

Aircraft Disinsection: What Aircraft Operators Need to Know

Written by: Pilots HQ Staff

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Time to read 6 min

In today’s global aviation environment, ensuring the health and safety of passengers, crew, and ecosystems goes beyond cabin service and flight operations—it includes strict adherence to aircraft disinsection protocols. With growing concerns over the international spread of vector-borne diseases and invasive pests, many countries now require inbound flights to perform disinsection using approved insecticide methods. For airline operators, procurement teams, and aviation safety officers, understanding these requirements—and sourcing EPA-registered, WHO-compliant solutions—is essential to maintaining compliance, avoiding penalties, and safeguarding public health.

Understand what aircraft disinsection is and why it’s required globally

Explore WHO-approved methods like pre-spray, top of descent, and cargo treatments

Learn which disinsection methods are EPA-registered and allowed in the U.S.

See how Callington’s Pre-Spray and 1-Shot products ensure safe, compliant treatment

Get guidance on where to find country-specific disinsection regulations

Know the consequences of non-compliance, including fines and denied entry

Why Aircraft Disinsection Matters

Global aviation doesn’t just move people and cargo—it can also inadvertently transport pests and disease vectors across borders. That’s why aircraft disinsection is a critical part of international aviation protocols.


Disinsection is the process of eliminating insects on board an aircraft using approved insecticides. It’s often mandated by countries to protect their ecosystems and public health from pests that may carry diseases such as:

  • Malaria
  • Zika virus
  • Yellow fever
  • Dengue
  • Agricultural pests

Aircraft disinsection is regulated by global health bodies, such as the World Health Organization (WHO), and aviation authorities, such as the International Civil Aviation Organization (ICAO). Additionally, individual countries enforce their own entry requirements, making compliance a complex yet essential task for airlines and operators.

Close-up of a mosquito feeding on human skin, highlighting the risk of disease transmission and the importance of aircraft disinsection protocols.
Adobe Stock

✈️ What Is Aircraft Disinsection?

According to the WHO, aircraft disinsection refers to treating an aircraft’s interior, cargo hold, and/or passengers’ cabin with insecticides to eliminate or prevent the entry of insects. There are several approved methods:

Common Aircraft Disinsection Methods

  1. Pre-embarkation spraying (Pre-Spray)

    • Applied before passengers board.

    • Used inside the cabin for residual effect.


  2. Top of descent spraying

    • Performed in the last 30 minutes before landing.

    • Applied while passengers are on board.

    • Not currently approved by the EPA for use in the U.S.


  3. Blocks-away spraying

    • Sprayed after doors are closed but before taxiing.

    • Used primarily in flights to Australia and New Zealand.


  4. Residual treatment

    • Conducted on aircraft interiors at set intervals.

    • Effective for multiple flights over a fixed period.


Flight attendant performing aircraft disinsection by spraying insecticide aerosols in an airplane cabin, following international health protocols.
https://www.callington.com/

5. Cargo hold aircraft disinsection (1-Shot)

  • Applied in the cargo area before loading.

  • EPA-approved for use in U.S.-originating flights.

Bold compliance isn’t optional—countries like Australia, New Zealand, and South Africa can fine or even refuse entry to aircraft that haven’t followed their aircraft disinsection protocols.

EPA Registration and Why It Matters in the U.S.

In the United States, all pesticide products must be registered with the Environmental Protection Agency (EPA) to be legally used.

Why this matters:

  • Only EPA-registered insecticides can be used on U.S.-originating flights.

  • U.S. operators must ensure they’re using approved aircraft disinsection products, or risk regulatory penalties and operational delays.

  • Top-of-descent sprays are NOT currently registered by the EPA for use while passengers are onboard, limiting their availability in the U.S. market.

At Pilotshq.com, we offer two EPA-registered solutions from Callington—a global leader in aviation-grade chemical products:

Both products are non-flammable, WHO-compliant, and approved for use on aircraft.


🇺🇸 Disinsection Methods Allowed Under U.S. EPA Regulations


In the United States, Pre-Embarkation is the only aircraft disinsection method currently approved by the EPA.

This method requires:

  • Callington Pre-Spray – Applied in the cabin before passengers board
  • Callington 1-Shot Cargo Hold Spray – Used in the cargo hold before or after loading

Not Approved by the EPA for U.S. Use (Any method with passengers onboard):

  • Residual disinsection methods
  • Pre-flight spraying
  • Top-of-descent spraying

These methods may be approved internationally but are not legally allowed on U.S.-based aircraft without explicit EPA approval.

⚠️ Compliance Reminder: Always verify your destination’s disinsection requirements and ensure you’re using EPA-registered products to remain compliant and avoid delays or penalties.

How to Check Country-Specific Disinsection Requirements

Airlines flying internationally must ensure aircraft disinsection is conducted in accordance with each destination country’s regulations.

Where to Find Reliable Disinsection Requirements

Here are the top official sources for up-to-date information:


1. WHO Disinsection Guidelines

2. ICAO Annex 9 – Facilitation

  • ICAO’s standards on international aircraft disinsection practices and documentation.

  • Includes procedures for customs and public health compliance.

  • Visit ICAO Annex 9

3.  IATA Traveler’s Health and Disinsection Page

  • IATA tracks which countries require aircraft disinsection and what methods are acceptable.

  • Check IATA guidelines

4.  Destination Country Websites

Examples:

  • Australia – agriculture.gov.au

  • New Zealand – mpi.govt.nz

  • South Africa – Department of Health portal

  • India, Indonesia, China – Check each country’s Ministry of Health or Aviation Authority

5. Other Useful Links on Aircraft Disinsection:


Important: Requirements can change seasonally (e.g., during mosquito outbreaks), so verify before every route update.

World Map of Aircraft Disinsection requirments
https://nbaa.org/aircraft-operations/international/customs-and-regulatory-issues/aircraft-disinsection/

Disinsection Products Airlines Can Trust

When selecting an aircraft disinsection product, look for:

  • ✅ WHO-compliant ingredients (e.g., permethrin, d-phenothrin)

  • ✅ Aircraft material compatibility (non-flammable, no residue)

  • ✅ EPA registration for U.S.-based operators

  • ✅ Approval from Airbus/Boeing Consumables Lists


Why Choose Callington’s Aircraft Insecticides?

Callington is a trusted name in aviation-grade chemicals, with disinsection products approved for:

  • Commercial airlines

  • Cargo aircraft

  • Military aircraft

  • Charter services

Their insecticides offer:

  • Quick knockdown and residual protection

  • No damage to aircraft interiors

  • WHO and ICAO compliance

  • EPA registration (for Pre-Spray and 1-Shot)

Use Callington for safe, compliant, and effective aircraft disinsection.

What is aircraft disinsection?

Aircraft disinsection is the process of spraying insecticides inside aircraft cabins or cargo areas to eliminate or prevent the entry of insects that may carry diseases or damage agriculture.

Is aircraft disinsection mandatory for all international flights?

No. Disinsection is only required for flights entering specific countries such as Australia, New Zealand, India, and several African and Caribbean nations. Each country has its own regulations.

Who sets the global standards for aircraft disinsection?

The World Health Organization (WHO), International Civil Aviation Organization (ICAO), and International Air Transport Association (IATA) provide guidelines followed by most national authorities.

What methods of aircraft disinsection are approved?

Standard methods include pre-embarkation spraying, residual treatments, cargo hold spraying, and in some cases, top of descent spraying (just before landing).

Are all insecticides approved for use on aircraft?

No. Only insecticides that are aircraft-safe, non-flammable, and approved by aviation authorities and regulatory bodies like the EPA and WHO can be used.

What does it mean if a product is EPA-registered?

EPA registration ensures the insecticide has been evaluated for safety and efficacy and is approved for its specific use in the U.S.—such as cargo or cabin disinsection.

Which Disinsection Methods Are Allowed on U.S.-Based Flights?

The only aircraft disinsection method currently approved in the USA is the Pre-Embarkation Method.

This method requires:

  • Callington Pre-Spray for cabin treatment before boarding

  • Callington 1-Shot for disinsection of cargo holds (if applicable)

Residual, Pre-Flight, and Top-of-Descent aircraft disinsection methods (Any method with passengers onboard)  are not currently approved for use in the U.S. by the EPA.


How can I find out what disinsection procedures are required for a specific country?

You can check the WHO guidelines, IATA’s Traveler’s Health page, or the official website of the destination country’s Ministry of Health or Agriculture.

What are the consequences of non-compliance with aircraft disinsection rules?

Operators may face fines, delayed aircraft clearance, or even denial of entry if aircraft disinsection requirements are not met upon arrival.

Which aircraft disinsection products are recommended for compliance?

We recommend Callington Pre-Spray for cabin disinsection and Callington 1-Shot for cargo hold treatments—both are WHO-compliant and EPA-registered for use in the U.S.

Stay Compliant, Stay Flying

Aircraft disinsection is more than a regulatory checkbox—it’s a vital part of protecting public health, biodiversity, and aviation reputations.


With global travel rebounding, countries are doubling down on biosecurity, and non-compliance can lead to delays, fines, or denied entry.


Equip your fleet with the right products, and stay updated on the evolving aircraft disinsection landscape. When in doubt, trust EPA-registered, WHO-compliant solutions from Callington, available now at Pilotshq.com.

Pilots HQ Staff

The Pilots HQ staff is a dedicated team of aviation supply specialists committed to supporting pilots, airlines, and aviation professionals with top-quality gear and regulatory insights. With years of industry experience, we provide trusted knowledge and reliable products—from uniforms to FAA- and EPA-compliant aircraft essentials. At Pilots HQ, we don’t just supply aviation—we support the people who make it fly.